Ms. Ammara Masood

Ms. Ammara Masood

Lecturer

Faculty of Life Sciences

Ms. Ammara Masood completed her M-Phil (Biotechnology) from National Institute for Biotechnology and Genetic Engineering (NIBGE). Her research focusses on hybrid gene promoter cloning using bioinformatics as well as molecular biology tools. Her current assignments includes the evaluation and identification of hypothyroid and congenital thyroid diseases and exploring its potential in Pakistani population using bio statistical tools. She has participated in various national and international conferences/seminars both as speaker as well as organizer. She has published 9 international full length research publications and 6 international abstracts. Her research expertise are isolation and cloning of promoters, vector construction of different cloning vectors, molecular plant breeding and comparative genomics analysis, cell culture & transfection, gene gun methods, analysis and interpretation of the data results through translational bioinformatics.

  1. BIOSTATISTICS
  2. PROBABILITY AND BIOSTATISTICS
  3. AGRICULTRAL BIOTECHNOLOGY
  4. HUMAN PHYSIOLOGY

Educational Qualifications

M-phil. (Biotechnology)

(Molecular Biology and Biotechnology)National Institute for Biotechnology and Genetic Engineering Faisalabad (NIBGE-PIEAS) (2009-2011)

BS Hons. (Bioinformatics)

Government College University Faisalabad (GCUF) (2004-2008)

Technical Skills

High level of skill in design and use of information techniques with emphasis on the organization, display and analysis of complex biological data.

  • Strong Concepts & Logics: Biotechnology and its applications, Techniques in biotechnology, Recombinant DNA technology, Molecular biology, Data Analysis in bioinformatics.
  • Tools: CLC bio, Net Primer, Primer 3,webcutter, KOBAS, JustBIO, Oracle 8.0.1 Enterprise, MySQL, MS Visio, MATLAB, SRSS.
  • Skills: Screening and Characterization of promoter sequences using biological tools.

Areas of interest

  • Interpretation of data using statistical tools.
  • Isolation and cloning of promoters using molecular biology techniques.
  • Vector construction of different cloning vectors.
  • Protein expression, isolation and purification.
  • Denaturing/Non-denaturing and gradient PAGE .
  • Gel documentation System.
  • Molecular plant breeding.
  • Comparative genomics.
  • Cell culture & Transfection.
  • Enzyme Linked Immuno Sorbent Assay (ELISA).
  • Polymerase Chain Reaction (PCR).
  • Microbiological Assay.
  • Analysis and interpretation of the data results through translational bioinformatics.

Assessment tests

  • Graduate Assessment Test (GAT)-General conducted by National Testing Service (NTS) of Pakistan on January 5, 2014.
  • Graduate Assessment Test (GAT)-subject Biotechnology conducted by National Testing Service (NTS) of Pakistan on May 18, 2014.
  • Graduate Assessment Test (GAT) – Subject test (Biotechnology) conducted by National Institute for Biotechnology & Genetic Engineering (NIBGE), 2009.

Passed Cambridge English entry level certificate in ESOL international (Business). Council of Europe level B1,September 2014

Projects

Current project as researcher in LUMS

“Evaluation of Thyroid disorder (patient dataset analysis) using biostatistics and translational bioinformatics”

Final Project of M-Phil Biotechnology

  • “Isolation and Evaluation of some monocot promoters”

Final Project of BS(Hons) Bioinformatics

  • “Comparative Genomic Analysis of Escherichia Coli Genome w.r.t its Virulence Nature”

Workshops and Symposiums

  • Attended a one day symposium on “DNA barcoding: a promising tool for species identification and biodiversity digitization”. Organized at NIBGE, dated: 30-01-12.
  • Attended a three day workshop on “ molecular diagnosis of dengue virus” organized at NIBGE from 1-3rd Feb, 2012.
  • Attended a 2 day workshop on “1st international conference on bioenergy, challenges and opportunities in Pakistan” November 20-21,2014. University of south asia Lahore.
  • Attended a 2 day workshop on “2nd international conference on biotechnology, challenges and opportunities in Pakistan” February 24-25,2014. University of south asia Lahore.
  • Attended a 2 day workshop on “3rd international conference on biotechnology, challenges and opportunities in Pakistan” February 8-9,2017. University of south asia Lahore.

Work Experience

  • Working as a researcher fellow in bioinformatics laboratory in LUMS.
  • Teaching experience in Aitchison college.
  • Worked as an editor of African journal of biotechnology.

Publications

  • “In silico comparative genomic analysis of Escherichia coli E24377A and Escherichia coli HS”. 2016. Ammara Masood*, Hira Mubeen and Nadia Iqbal. International Journal of Medical and Health Research (ijmhr). 2(3) 3-10.
  • “Cloning of D-Hordein hybrid promoter from Hordeum Vulgare and its expression analysis.” 2017. Ammara Masood*, Nadia Iqbal, Hira Mubeen, Rubab Zahra Naqvi, Asia Khatoon, and Aftab Bashir. Pakistan journal of botany. Pak .J. Bot., 1085-1095.
  • “Cloning and expression analysis of alcohol dehydrogenase hybrid promoter isolated from zeamays”. Ammara Masood*, Nadia Iqbal, Hira Mubeen, Rubab Zahra Naqvi, Asia Khatoon, and Aftab Bashir. African Journal of Biotechnology. 15(42): 2384-2393.
  • “Isolation and characterization of SPS promoter from cotton”. 2017. Nadia Iqbal1*, Muhammad Asif, Amara Masood, Rubab Zahra Naqvi, Asia Khatoon, Aftab Australian journal of crop sciences. AJCS 11(6):668- 675.
  • Histochemical GUS expression of cotton SPS promoter in transgenic tobacco. Nadia Iqbal, Ammara Masood, Aftab Bashir and Mohammad Asif. 2016. Journal of advances in biology and biotechnology ( JABB). 8(1): 1-8.
  • “In silico mutation analysis of human Beta globin gene in SCD patients”.2016. Hira Mubeen, Rubab Zahra, Ammara Masood. International Journal of Research in Medical Sciences. 4(5):1673-1677.

 

  • Review paper on “Gene transformation, method uses and application”. Hira Mubeen, Ammara Masood and Rubab Zahra Naqvi. 2016. Journal of pharmaceutical and biological sciences.
  • “Cloning and expression analysis of alcohol dehydrogenase hybrid promoter isolated from zeamays”. Ammara Masood, Nadia Iqbal, Hira Mubeen, Rubab Zahra Naqvi, Asia Khatoon, and Aftab Bashir. African Journal of Biotechnology. 15(42): 2384-2393.
  • Histochemical GUS expression of beta tubulin promoter in transgenic tobacco. Hira Mubeen, Aftab Bashir, Ayesha Ameen, Ammara Masood and Shahid Raza. African Journal of Biotechnology. 16(17): 945-952.
  • “Identification, isolation and evaluation of a constitutive sucrose phosphate synthase gene promoter from tomato. 2017. Hira Mubeen, Rubab Zahra Naqvi, Ammara Masood, Asia Khatoon, and Aftab Bashir. Pakistan journal of botany. Pak.J.Bot.49 (3):1105-1112.
  • “Isolation and characterization of SPS promoter from cotton”. 2017. Nadia Iqbal1*, Muhammad Asif, Amara Masood, Rubab Zahra Naqvi, Asia Khatoon,              Aftab Australian journal of crop sciences. AJCS 11(6):668- 675.
  • Isolation and characterization of sps promoter from cotton.2016. Submitted to Asian journal of applied sciences (ajsc)5.3.313 ,reference # 1607.

“Revealing expression of resistance genes homologues against cotton leaf curl virus in gossypium arboreum and gossypium hirsutum cotton genotypes,” Submitted and is under review in the journal of animal and plant sciences (IF 0.422) under accession number AG-17-0097.

Participated in conference

  • Abstract publication in “2nd international conference on biotechnology (ICB): challenges and oppurtunities in Pakistan.” (February 24-25, 2016). Submitted in USA journal of R& D. ISSN:2412-6926.
  • Attended “National Conference on Commercialization of Biotechnology Products.
  • Actively precipitated in 28th Pakistan Congress of Zoology held at GCU Faisalabad, March 18-20, 2008.
  • 2nd April 2008 to 30th May 2008 -Internship at NIBGE(National Institute for Biotechnology & Genetic Engineering ) Faisalabad.
  • Poster presentation entitled “Cloning of D-Hordein hybrid promoter from Hordeum Vulgare and its expression analysis”.  At 2nd International Conference on Biotechnology (ICB)  held at University of South Asia, Lahore, 24-25 February, 2016.

ammara.masood@ucp.edu.pk
+92-42-35880007 Ext:
Faculty of Life Sciences, University of central Punjab

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